Correlated Study of Dengue Serological markers and Platelet count

  • Asim Kumar Singh Department of Microbiology, HIMS, Varanasi.
  • Saumya Singh Department of Microbiology, HIMS, Varanasi.
  • Mukesh Kumar Singh Department of Microbiology, HIMS, Varanasi.
  • Mahak Jain Department of Microbiology, HIMS, Varanasi.
  • Nisha Chaitanya Assistant professor, Microbiology, Bharati Vidyapeeth Deemed University Medical College and Hospital, Sangli, Maharashtra
Keywords: Thrombocytopenia, dengue, NS1 antigen, seropositive, dengue serological markers.


Background: Dengue being the most prevalent mosquito borne arboviral infection in India, has become endemic in India with every year outbreaks. Despite substantial efforts to control the mosquito populations, dengue fever has spread, emerged and established itself rapidly. 

Objectives: This study is to correlate the platelet count and IgM /IgG and NS1 in the acute stage of dengue infection

Materials & Methods: The present study was conducted for a period of 22 months in Heritage Institute of Medical Sciences from January 2016 to November 2017. Blood samples were collected from 1347 suspected Dengue patients. In all the serologically positive cases, serological confirmation and evaluation of platelet counts of dengue infection was done.

Results: A total of 1347 suspected cases were admitted during the study period and among them, 155 (11.51%) were found to be seropositive for dengue. Among the dengue cases, Males (61.05%) were affected more than females (38.7%). Out of 155 positive cases, 108 [69.7%] cases were positive for NS1antigen either alone or in combination with antibodies.77 [49.7%] cases were exclusively positive for NS1 antigen only. Out of 108 cases that were positive for NS1, thrombocytopenia was observed in 70 cases (64.8%) whereas when the antibodies alone were considered, thrombocytopenia was observed in 18 out of 47 cases (38.3%. In a total of 155 cases, thrombocytopenia was seen in 88 cases (56.8%).

Conclusion: The study draws attention toward diagnosis of dengue serologically by testing NS1 antigen and IgM / IgG antibodies. NS1inclusion in the diagnosis of dengue increases the chance of early diagnosis in order to avoid complications significantly


Download data is not yet available.


1. Moorthy M, Chandy S, Selvaraj K, Abraham AM. Evaluation of a rapid Immnunochromatoghraphic device for the detection of IgM and IgG antibodies to dengue viruses (DENV) in a tertiary care hospital in south India. Indian J Med Microbiol 2009;27:254 256.
2. Young PR, Hilditch PA, Bletchly C, Halloran W. An antigen capture enzyme linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 2000;38:1053 1057.
3. Martina BE, Koraka P, Osterhaus AD. Dengue virus pathogenesis: An integrated view. Clin Microbiol Rev 2009;22:564 581.
4. Ho TS, Wang SM, Lin YS, Liu CC. Clinical and laboratory predictive markers for acute dengue infection. J Biomed Sci 2013;20:75.
5. Guzmán MG, Kourí G. Dengue: An update. Lancet Infect Dis 2001;2: 33 42.
6. Libraty DH, Young PR, Pickering D, Endy TP, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis 2002;186:1165 1168.
7. 7.World Health Organization. Dengue haemorrhagic fever: Diagnosis, treatment, prevention and control. 2 nd edition. Geneva, Switzerland: Chapter 2, Clinical Diagnosis, 1997:12-23.
8. 528-529 Jawetz,Melnick & Adelberg’s Medical Microbiology 25th edition 2001.
9. Datta S, Wattal C. Dengue NS1 antigen detection: A useful tool in early diagnosis of dengue virus infection. Indian J Med Microbiol 2010;28:107-110.
10. World Health Organization. 1997. Dengue haemorrhagic fever: Diagnosis, treatment, prevention and control. 2nd edition. Geneva, Switzerland. Chapter 4, Laboratory Diagnosis.
11. Subedi D and Taylor-Robinson AW. Laboratory diagnosis of dengue infection: current techniques and future strategies. Open Journal of Clinical Diagnostics. 2014; 4: 63-70.
12. Apurba sankar sastry, Sandhya Bhat K. Essentials of Medical Microbiology.2016;(1):486-491.
13. Young PR, Hilditch PA, Bletchly C, Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol.2000;38:1053–1057.
14. Dengue: Guidelines for diagnosis, treatment, prevention and control. New edition Geneva: World Health Organization. 2009.
15. Ukey PM, Bondade SA, Paunipagar PV, Powar RM, Akulwar SL. Study of seroprevalence of dengue fever in central India. Indian J Community Med. 2010;35(4):517-519.
16. Saini S, Kinikar AG, Deorukhkar S, Bhalerao D, Roushani SB. Epidemiology and seropositivity of dengue fever cases in a rural tertiary care hospital of western Maharashtra, India. Int J Bio Med Res.2013;4(7):473-477.
17. Sood S. A hospital based serosurveillance study of dengue infection in Jaipur (Rajasthan). India. J Clin Diagn Res. 2013;7(9):1917-1920.
18. Rao MS, Pavani K, Dass M, Kareem MA, Vinayaraj EV. Seroprevalence of dengue virus in a tertiary care hospital, Andhra Pradesh, South India. Int J Res Med Sci. 2013;1(4):448-450.
19. Karoli R, Fatima J, Siddiqi Z, Kazmi K, Sultania A. Clinical profile of dengue in India. J Infect Dev Ctries. 2012;6(7):551-554.
20. Murugananthan K, Kandasamy M, Rajeshkannan N, Noordeen F. Demographic and clinical features of suspected dengue and dengue haemorrhagic fever in the Northern Province of Sri Lanka, a region afflicted by an internal conflict for more than 30 years-a retrospective analysis. Int J Infect Dis. 2014; 27:32-36.
21. Halstead SB, Nimmannitya S, Cohen SN. Observations related to pathogenesis of dengue hemorrhagic fever. IV. Relation of disease severity to antibody response and virus recovered. Yale J Biol Med. 1970;42(5):311-328.
22. RD Kulkarni, SS Patil, GS Ajantha, AK Upadhya, AS Kalabhavi, RM Shubhada, et al.; Association of platelet count and serological markers of dengue infection importance of NS1 antigen. Indian Journal of Medical Microbiology, 2011; 29(4): 359-362.
23. Santosh Shivajirao Tathe*, Chincholkar VV, Kulkarni DM, Nilekar SL, Ovhal RS, and Halgarkar CS. A study of NS1 antigen and platelet count for early diagnosis of dengue infection. Int.J.Curr. Microbiol. App. Sci (2013) 2(12): 40-44
24. Khan MU, Rehman R, Gulfraz M, Latif W. Incidence of thrombocytopenia in seropositive dengue patients. Int J Med Sci. 2014;6(4):113-116.
25. Khan DM, Kuppusamy K, Sumathi S, Mrinalini VR. Evaluation of thrombocytopenia in dengue infection along with seasonal variation in rural Melmaruvathur. J Clin Diagn Res. 2014;8(1):39-42
26. Kauser M, Kalavathi GP, Radadiya M, Karthik M, Afreen Afreen A, Kumaraswamy RC, Va M. A study of clinical and laboratory profile of Dengue fever in tertiary care hospital in central Karnataka, India. Global J Med Res. 2014;14(5).
27. Ghosh K, Gangodkar S, Jain P, Shetty S, Ramjee S, Poddar P, et al. Imaging the interaction between dengue 2 virus and human blood platelets using atomic force and electron microscopy. J Electron Microsc. 2008;57(3):113-118.
28. Saroj Golia, Vivek Hittinahalli, K. Sujatha Karjigi, Mallika Reddy, Asha S. Kamath. Serodiagnosis of dengue using NS1 antigen, dengue IgM, dengue IgG antibody with correlation of platelet counts. International Journal of AJ Institute of Medical Sciences (2012);1(2):112-117.
How to Cite
Singh AK, Singh S, Singh MK, Jain M, Chaitanya N. Correlated Study of Dengue Serological markers and Platelet count. Int Arch BioMed Clin Res [Internet]. 2018Mar.21 [cited 2022Dec.4];4(1):137-40. Available from: